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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/12/2005 |
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Data da última atualização: |
21/08/2009 |
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Autoria: |
GASPAR, J. O.; BELINTANI, P.; ALMEIDA, A. M. R.; KITAJIMA, E. W. |
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Título: |
A primer pair allows amplification of part of the 3'-terminal of carlaviruses genome. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Virus Reviews & Research, Rio de Janeiro, v. 10, p. 140, Nov. 2005. Supplement, ref. P-191. |
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Idioma: |
Inglês |
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Notas: |
Edição dos Resumos da XVI National Meeting of Virology, nov. 2005. |
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Conteúdo: |
The genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP gene showed the highest identify (78,3% nt and 93,5% aa) with the isolate H of Cowpea mild mottle virus (AF024628). Our designed primer have the advantage that about 930 nt from the 3' terminus, comprising part of the CP gene, the 11K gene and the terminal untranslated region can be amplified for sequencing, allowing the detection of carlaviruses and identification of unknown viruses as mebers of this virus genus. MenosThe genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP ge... Mostrar Tudo |
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LEADER 02495naa a2200169 a 4500
001 1468611
005 2009-08-21
008 2005 bl --- 0-- u #d
100 1 $aGASPAR, J. O.
245 $aA primer pair allows amplification of part of the 3'-terminal of carlaviruses genome.
260 $c2005
500 $aEdição dos Resumos da XVI National Meeting of Virology, nov. 2005.
520 $aThe genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP gene showed the highest identify (78,3% nt and 93,5% aa) with the isolate H of Cowpea mild mottle virus (AF024628). Our designed primer have the advantage that about 930 nt from the 3' terminus, comprising part of the CP gene, the 11K gene and the terminal untranslated region can be amplified for sequencing, allowing the detection of carlaviruses and identification of unknown viruses as mebers of this virus genus.
700 1 $aBELINTANI, P.
700 1 $aALMEIDA, A. M. R.
700 1 $aKITAJIMA, E. W.
773 $tVirus Reviews & Research, Rio de Janeiro$gv. 10, p. 140, Nov. 2005. Supplement, ref. P-191.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 56 | |
| 25. |  | CARVALHO, C. J. R. de; DAVIDSON, E. A.; SANTOS, T. P. dos; DUTRA, F. C.; SERRÃO, B. de O. Changing enzymatic activities and mycorrhizal infections in a chronosequence of secondary and mature forests of eastern Amazonia. In: CONFERÊNCIA CIENTÍFICA DO LBA, 3., 2004, Brasília, DF. Anais de trabalhos completos. Brasília, DF: LBA, 2004. Resumo 23.1.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 26. | | MARKEWITZ, D.; FIGUEIREDO, R. de O.; DAVIDSON, E. A.; BUSTAMANTE, M. M. C.; PARRON, L.; RESENDE, J. The chemistry of two streams draining kaolinitic soils. In: CONFERÊNCIA CIENTÍFICA DO LBA, 3., 2004, Brasília, DF. Anais de trabalhos completos. Brasília, DF: LBA, 2004. Plenária 6. Resumo 20.5.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 27. | | MARKEWITZ, D.; RESENDE, J. C. F.; PARRON, L.; BUSTAMANTE, M.; KLINK, C. A.; FIGUEIREDO, R. de O.; DAVIDSON, E. A. Dissolved rainfall inputs and streamwater outputs in an undisturbed watershed on highly weathered soils in the brazilian cerrado. Hydrological Processes, v. 20, n. 12, p. 2615-2639, Aug. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 28. | | RANGEL-VASCONCELOS, L. G. T.; FIGUEIREDO, R. de O.; SCHULER, M. A. E.; MARKEWITZ, D.; DAVIDSON, E. A. Integration of biogeochemical and hydrological data in pasture and forest covered catchments in Eastern Amazonia. In: SCIENCE TEAM MEETING, 10., 2006, Brasília, DF. Book of Abstracts... Manaus: LBA-ECO, 2006. p. 49.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 29. | | SILVA, P. de S.; FIGUEIREDO, R. de O.; CUNHA, E. da S.; SCHULER, M.; MARKEWITZ, D.; DAVIDSON, E. A. Influência do uso da terra sobre a qualidade das águas de quatro igarapés na Amazônia Oriental: variabilidade espacial e sazonal de pH, condutividade elétrica, oxigênio dissolvido, temperatura, e turbidez. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 2., 2005, Manaus. Resumos... [S.l.]: LBA, [2005?]. p. 45.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 32. | | FREITAS, J. F. B. de; SÁ, T. D. de A.; ISHIDA, F. Y.; SABÁ, R. T.; DAVIDSON, E. A.; FIGUEIREDO, R. de O. Alterações nas emissões de gases traço em função da substituição do sistema de produção agrícola de corte/queima pelo de corte/trituração/manejo de capoeira. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA FCAP, 12.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 6., 2002, Belém, PA. A contribuição do profissional de Ciências Agrárias no uso e conservação da biodiversidade: anais. Belém, PA: FCAP: Embrapa Amazônia Oriental, 2002.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 33. | | CARVALHO, C. J. R. de; DAVIDSON, E. A.; SANTOS, T. P. dos; FIGUEIREDO, R. de O.; SERRÃO, B. de O.; DUTRA, F. C. Atividade da fosfatase ácida, uréase e micorrizas em uma área de vegetação secundária de Paragominas, Estado do Pará, dois anos após adubação com nitrogênio e fósforo. In: CONFERÊNCIA CIENTÍFICA DO LBA, 3., 2004, Brasília, DF. Anais de trabalhos completos. Brasília, DF: LBA, 2004. Plenária 6. Resumo. Resumo 28.9-P.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 34. | | VIEIRA, I. C. G.; ALMEIDA, A. S. de; DAVIDSON, E. A.; STONE, T. A.; CARVALHO, C. J. R. de; GUERRERO, J. B. Classifying successional forests using landsat spectral properties and ecological characteristics in eastern Amazônia. Remote Sensing of Environment, v. 87, n. 4, p. 470-481, 2003. il.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 35. | | ALMEIDA, A. S. de; VIEIRA, I. C. G.; DAVIDSON, E. A.; STONE, T. A.; CARVALHO, C. J. R. de; GUERRERO, J. B. Classifying successional forests using landsat spectral properties and ecological characteristics to evaluate recent trends in land cover and carbon loss in eastern Amazonia. In: CONFERÊNCIA CIENTÍFICA INTERNACIONAL DO LBA, 2., 2002, Manaus. Resumos. Manaus: LBA, 2002. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 36. | | SERRÃO, B. de O.; DUTRA, F. C.; SANTOS, M. T. P. dos; SOUZA, C. M. de A.; TRINDADE, I. A.; CARVALHO, C. J. R. de; DAVIDSON, E. A. Carbono e nitrogênio na biomassa microbiana do solo de uma área de pastagem abandonada, três anos após fertilização com N e P. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 2., 2005, Manaus. Resumos... [S.l.]: LBA, [2005?]. p. 45.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 37. | | SIDDIQUE, I.; VIEIRA, I. C. G.; SCHMIDT, S.; LAMB, D.; CARVALHO, C. J. R. de; FIGUEIREDO, R. de O.; DAVIDSON, E. A. Early-successional dynamics of tree species composition in relation to nitrogen and phosphorus supply in degraded pasture in Eastern Amazonia. In: ESAR/SER JOINT MEETING, 2007, San Jose, California. Abstract. San Jose: San Jose State University, 2007. Abstract ID: 4655. Password: 290749.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 38. | | PACHECO JÚNIOR, A. C.; SILVA, P. S.; FIGUEIREDO, R. de O.; CUNHA, E. da S.; SCHULER, M.; MARKEWITZ, D.; DAVIDSON, E. A. Estudo estatístico preliminar de parâmetros físico-químicos e biogeoquímicos das águas do Igarapé 54 em Paragominas/PA - Amazônia Oriental. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 2., 2005, Manaus. Resumos... [S.l.]: LBA, [2005?]. p. 85.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 39. | | CARVALHO, C. J. R. de; CHU, E. Y.; DAVIDSON, E. A.; SANTOS, M. T. P. dos; FIGUEIREDO, R. de O.; FREIRE, G. S.; PANTOJA, K. de F. R. Mechanisms of conservation and cycling of N and P in a chronosequence of secondary vegetation in Eastern Amazonia. In: CONFERÊNCIA CIENTÍFICA INTERNACIONAL DO LBA, 2., 2002, Manaus. Resumos... Manaus: LBA, 2002. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 40. | | SCHULER, M. A. E.; MORAES, J. M. de; DUNNE, T.; FIGUEIREDO, R. de O.; MARKEWITZ, D.; DAVIDSON, E. A.; VICTORIA, R. L. Hydrological processes in small forest and pasture catchments of the eastern amazônia. In: CONFERÊNCIA CIENTÍFICA DO LBA, 3., 2004, Brasília, DF. Anais de trabalhos completos. Brasília, DF: LBA, 2004. Plenária 6. Resumo 20.2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 56 | |
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